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. 2023 Apr 5;34(7):1191–1206. doi: 10.1681/ASN.0000000000000126

Figure 3.

Figure 3

Rab7 KO mice podocytes accumulate unprocessed late endosome-like vesicles (ULLVSs). (A–F) Ultrastructural analyses (TEM close up) of 2–3-week-old, 4-week-old, and 8-week-old mice with a focus on FP and SD; 2–3-week-old Rab7fl/fl;PodCre+ mice seem healthy. Four-week-old Rab7fl/fl; PodCre+ mice show first signs of FPE and a slightly thickened GBM (white arrow). Eight-week-old mice show FPE and SD disruption. (G/H) TEM analyses of glomeruli of 8-week-old mice. Rab7-depleted podocytes show disrupted SDs and FPE. (I–K) TEM analyses of 2–3-week-old mice with a focus on accumulating vesicles within the podocyte (white arrow), named ULLVs (unprocessed late endosome-like vesicles). (L–N) TEM analyses: Detailed view on accumulating vesicles; accumulating double membrane structures are marked with black arrowheads, large single membrane structures with white arrowheads. B, blood vessel; FP, foot process; FPE, foot process effacement; GBM, glomerular basement membrane; N, nucleus; Pod, podocyte body; SD, slit diaphragm; TEM, Transmission Electron Microscopy; ULLVs, unprocessed late endosome-like vesicles.