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Summary of reactions catalyzed by purified DoxA and their respective kcat/Km values expressed in M−1 · s−1. DOC, 13-deoxycarminomycin; DHC, 13-dihydrocarminomycin; CAR, carminomycin; DOD, 13-deoxydaunorubicin; DHD, 13-dihydrodaunorubicin; DAU, daunorubicin; DOX, doxorubicin; ND, not done (but previously shown to occur [9]). DauK (anthracycline 4-O-methyltransferase) catalyzes the methylation of 4-hydroxyanthracyclines to the 4-methoxy analogues.
