TABLE 5.
Bioconversion of DNR to DXR in the S. peucetius WMH1530 dnrN::aphII mutant and S. lividans TK23 transformed with pWHM546, pWHM547, pWHM548, or pWHM550
| Medium | Plasmid | DNR added (μg/ml) | DXR produced (μg/ml)b |
|---|---|---|---|
| GMSa | pWHM546 dnrUdnrVdoxA | 20 | 5 |
| pWHM547 ermE*::dnrVdoxA | 20 | 20 | |
| 100 | 50 | ||
| 200 | 142c | ||
| pWHM548 ermE*::doxA | 100 | 5 | |
| GPSd | pWHM546 | 20 | 1.5 |
| pWHM547 | 20 | 3 | |
| pWHM548 | 20 | <1 |
a
The host strain was S. peucetius WMH1530, and all strains were grown in the GMS medium (Materials and Methods). This work was performed at Pharmacia & Upjohn.
b
The data are the average values of two or three independent fermentations.
c
The host strain was S. lividans TK23, and work was performed at Pharmacia & Upjohn.
d
The host strain was S. peucetius WMH1530, and all strains were grown in GPS medium (Materials and Methods). This work was performed at the University of Wisconsin.