Estimated serum folate insufficiency threshold by vitamin B12 strata [n = 472 (<148 pmol/L), 255 (148–221 pmol/L), and 250 (≥221 pmol/L)].
Estimated serum folate concentrations corresponding to the RBC folate concentration of 748 nmol/L for optimal NTD prevention, by vitamin B12 status. The dashed lines correspond to the estimated median. Abbreviations: NTD, neural tube defect.