Extended Data Fig. 9. OviDN electrical activity during substrate transitions and additional evidence for oviDN ∆F/F tracking relative value during substrate transitions.

a, oviDN spike rate versus Vm at rest. b, Vm during two substrate transitions from the same fly. These sample traces have more pronounced Vm changes than is typical. c, Mean oviDN Vm after removal of spikes during substrate transitions (Methods). 74 and 72 traces from 8 cells in 8 flies (74 and 72 transitions). Light grey shading is ± s.e.m. for all panels in this figure. d, Mean oviDN spike rate during substrate transitions. e, Same as Extended Data Fig. 8a but for the behavior of the fly during this electrophysiology dataset. f, Mean oviDN ∆F/F during substrate transitions from 500 to 0 mM and 0 to 500 mM. 2459 and 2460 traces from 70 cells in 53 flies (1911 and 1922 transitions). g, Mean oviDN ∆F/F during substrate transitions from 500 to 200 mM and 200 to 500 mM. 167 and 170 traces from 5 cells in 3 flies (105 and 109 transitions). h, Mean oviDN ∆F/F during substrate transitions from 200 to 0 mM and 0 to 200 mM. 443 and 446 traces from 20 cells in 20 flies (443 and 446 transitions). In panels f-h, note that all changes are on the order of 0.05 ∆F/F regardless of the absolute sucrose concentration, consistent with a relative value calculation. i–k, Same as Extended Data Fig. 8a but for the behavior of the fly during the datasets in panels f-h, shown to the left.