FIG. 1.

Physical and genetic map of the eps gene cluster of plasmid pNZ4000. For BsaAI, EcoRI, HincII, NdeI, ScaI, and SstI, only sites relevant for subcloning are indicated. The plasmids used for heterologous and homologous expression of the eps genes are listed. Plasmids pNZ4060 through pNZ4065 are pUC19 derivatives carrying the indicated fragments under control of the lac promoter. Plasmids pNZ4070, pNZ4071, and pNZ4072 are pNZ8020 derivatives carrying the indicated fragments under control of the nisA promoter.