FIG. 2.

TLC of ¹⁴C-labelled intermediates isolated from the lipid fraction of permeabilized E. coli cells. The positions of the standard sugars glucose (Glc), galactose (Gal), and cellobiose (Glc-Glc), as well as those of the predicted trisaccharide (Glc-Glc-Gal) and the unknown galactose-containing molecule (?-Gal), are indicated on the right. Additional products of incomplete mild hydrolysis, putative sugar phosphates (A) and putative lipid-linked sugars (B), are indicated on the left. Lanes: 1 and 2, E. coli carrying pNZ4060; 3 and 4, E. coli carrying pNZ4061; 5 and 6, E. coli carrying pNZ4062; 7 and 8, E. coli carrying pNZ4063. Complete (C) and mild (M) acid hydrolysis treatments are indicated.