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. 2023 Jul 19;12:25. doi: 10.1186/s13619-023-00170-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — DSF treatment protects CMs from H₂O₂-induced injury in a dose-dependent manner. A-D Fold change of LDH release (A), CCK-8 detection (B), numbers of live CMs (C), and representative images of calcein AM staining (D) in NRVMs after exposure to H₂O₂ (400 μM) and different concentrations of DSF for 24 h. n = 6 (A, B), or n = 5 (C, D) per group. E–H Fold change of LDH release (E), CCK-8 detection (F), numbers of live CMs (G), and representative images of calcein AM staining (H) in NRVMs exposed to 400 μM H₂O₂ for 15 min, followed by treatment with different concentrations of DSF for 24 h. n = 6 (E, F), or 5 (G, H) per group. Scale bar, 100 μm (D, H). *p < 0.1, **p < 0.01, ***p < 0.001; ns, no significant difference. One-way ANOVA with Dunnett’s test (A-C, E–G). Data are the mean ± s.e.m. (A-C, E–G)