Figure 4.
Mean Wake Bout Durations in narcoleptic male Orexin-tTA;TetO-DTA mice (n = 7) treated with the HCRTR2 agonists TAK-925 (a) and ARN-776 (b). Hourly number of bouts of Wakefulness (c, d), NREM sleep (f, g), REM sleep (i, j), and Cataplexy (l, m) in narcoleptic mice treated with TAK-925 and ARN-776. Total number of bouts of Wake (e), NREM sleep (h), REM sleep (k) and Cataplexy (n) across the 6-h recording after treatment with TAK-925 and ARN-776. Values are mean ± SEM. Colored * above hourly graphs indicate significance (p < 0.05) during that hour relative to vehicle treatment. * in legend denotes significance for that treatment across the 6-h recording as determined by 2-way ANOVA. * in e and n denotes significance for that treatment relative to vehicle across the 6-h recording as determined by post hoc Holm-Sidak test. Abbreviations: Veh, vehicle; ZT, Zeitgeber Time.