Figure 2.

PCR screening for targeted clones. (A) Genomic structure of the wild-type, targeted, and deleted alleles. Black arrowheads indicate PCR primers. Other abbreviations are the same as in Fig. 1B. (B) Results of the PCR screening. The results in panels I, II, and III were obtained using the PCR primer pairs I, II, and III shown in (A), respectively. Bands close to the expected size are indicated by asterisks and bands longer than expected are indicated by arrowheads. Clones marked with arrows were re-cloned and analyzed as shown in (C). M DNA size marker. (C) PCR analysis after re-cloning. Bands surrounded by rectangles were purified and further analyzed by long-read sequencing. The original gels of (B) and (C) are presented in Supplementary Figs. 4 and 5, respectively.