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. 2023 Jul 19;13:11652. doi: 10.1038/s41598-023-38397-y

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Long-read sequence analysis of vector insertion. (A) Overview of long-read sequence analysis. (B) Example of annotation of a consensus sequence obtained by de novo assembly of long-read sequencing data. Examples of direct sequencing using the Sanger method, performed to confirm the fidelity of the assembly, are also shown. (C) Annotation of the results of Sanger sequencing in regions I, II, and III shown in (B).