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. 2023 Jun 1;17(8):1267–1277. doi: 10.1038/s41396-023-01428-7

Fig. 2. Microscopic analysis of Msb3 population dynamics after leaf inoculation.

Fig. 2

Visualization of bacteria by DOPE-FISH/CLSM microscopy. The FISH probe Burkho was applied to leaves of tomato. The lower epidermis is depicted at different timepoints: a 24, b 48 and c 72 hours post inoculation with a highly diluted bacterial suspension. Plant autofluorescence was visualized through the cyan channel, the Burkho probe through the magenta channel and the nucleic acid stain SYBR-Safe through the yellow channel. Panels a–f all show overlays of all three channels. Panels d, e and f show high-resolution images of the sections highlighted with a yellow frame in a, b and c, respectively.