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. 2023 Jul 19;14:4346. doi: 10.1038/s41467-023-40006-5

Fig. 1. Magnetic lifting maintains the structural integrity of neural organoids.

Fig. 1

a Diameter measurements of MSC and HUVEC spheroids, and hiPSC-derived ventral and dorsal forebrain neural organoids at increasing days of culture. Each data point represents a distinct spheroid or organoid (MSC n = 25, HUVEC n = 27, D25 Ventral n = 27, D50 Ventral n = 25, D100 Ventral n = 25, D25 Dorsal n = 25, D50 Dorsal n = 25, D100 Dorsal n = 12). p values for each diameter comparison are as follows: MSC vs. HUVEC p = 0.9978, all other shown comparisons p < 0.0001. b Mass measurements of spheroids and neural organoids. Each data point represents an average of five neural organoids. p values for each mass comparison are as follows: D25 Ventral vs. D50 Ventral p = 0.1499, D25 Ventral vs. D100 Ventral p = 0.0094, D50 Ventral vs. D100 Ventral p = 0.7933, D25 Dorsal vs. D50 Dorsal p < 0.0001, D25 Dorsal vs. D100 Dorsal p < 0.0001, D50 Dorsal vs. D100 Dorsal p = 0.4743. c Vacuum pressure required to lift neural organoids of increasing diameters within a liquid medium. Each data point represents a distinct organoid (1.0 mm n = 4, 1.5 mm n = 4, 2.0 mm n = 4, 2.5 mm n = 4). p values for minimum lifting pressure comparisons are as follows: 1.0 mm vs. 1.5 mm p = 0.0007, 1.5 mm vs. 2.0 mm p < 0.0001, 2.0 mm vs. 2.5 mm p = 0.0026. d Apparent surface tension of spheroids and neural organoids. Each data point represents a distinct spheroid or organoid (MSC n = 4, HUVEC n = 2, D25 Ventral n = 4, D50 Ventral n = 4, D25 Dorsal n = 4, D50 Dorsal n = 4). p values for apparent surface tension comparisons are as follows: MSC vs. HUVEC p = 0.0235, all other shown comparisons p < 0.0001. e Schematic of vacuum aspiration-assisted lifting of neural organoids. f Representative brightfield (BF) images of a neural organoid prior to vacuum aspiration. g Representative BF images of a neural organoid post vacuum aspiration (6 mmHg). h Representative BF image of a neural organoid that has undergone complete deformation (i.e., is no longer spherical) post vacuum aspiration (6 mmHg). i Quantification of the extent of deformation as a function of the applied vacuum pressure. Each color represents a single neural organoid (n = 3). j Representative quantification of neural organoid deformation during and immediately following vacuum aspiration (6 mmHg, shown in blue). k Long-term neural organoid deformation in response to two vacuum pressures: 6 mmHg and 10 mmHg. Each set of data points connected with a line represents a single biological replicate (6 mmHg n = 4, 10 mmHg n = 4). l Schematic of magnetic lifting of neural organoids. m Representative BF image of a neural organoid post magnetic lifting. Statistical analyses performed as one-way ANOVA with Tukey multiple comparisons test. Unless otherwise noted, all data points represent distinct biological replicates. Data plotted as mean ± SD where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and ns not significant.