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. 2023 Jul 19;13:11646. doi: 10.1038/s41598-023-38856-6

Figure 2.

Figure 2

G2-SUISO reduced the mRNA expression of inflammatory cytokine-related genes. (A) Homogenates of liver tissues were analyzed by qRT-PCR, as described in the Materials and Methods. The mRNA expression of inflammatory cytokine-related genes, particularly TNF-α, iNOS and CCR2, was significantly lower in the 3d_G2 group than in the 3d_control group. The mRNA expression of inflammatory cytokine-related genes, such as IFN-γ, OPN, IL-1β and IL-6, tended to be down-regulated following G2-SUISO treatment. Values are expressed as the mean ± SD in arbitrary units; *p < 0.05, **p < 0.01, ****p < 0.0001. (B) Chloroacetate esterase staining of liver specimens with inflammatory foci in the five groups is shown (magnification × 40 & × 200). (C) Analysis results of chloroacetate esterase staining of fatty liver specimens are shown. A total of 4 high power fields (hpf) (× 40) were randomly selected from each liver specimens (n = 5), and the number of inflammatory foci was counted. The data are expressed as the cell number/high-power field. Each bar represents the mean ± SD; *p < 0.05.