Fig. 2. Brain-pCLAP identifies known and novel RBPs.

a Locations of RNA-binding peptides identified by brain-pCLAP mapped to linear depictions of RBPs. Tissue expression data taken from brain atlas⁴⁸. b Schematic of membrane bound synaptic vesicle proteins identified as RNA-binding by brain-pCLAP, showing their trans-membrane domains as well as the locations of peptides identified by brain-pCLAP as RNA-binding regions (blue stars). c Data demonstrating RNA-binding ability of PGRMC1 and PGRMC2. Left: Volcano plots (as in Fig. 1c) with peptides from PGRMC1 or PGRMC2 marked in black. Middle: Schematic depiction of both proteins showing their trans-membrane and cytochrome b5-like domains and where the peptides identified by brain-pCLAP are located. Right: CLIP autoradiography for GFP-tagged PGRMC1 and PGRMC2, with visualization of the isotope labelled protein-RNA complex. The left lane is a no cross-link control, followed by the cross-linked samples from highest to lowest concentration of RNase (dilutions 1:10, 1:100 or 1:1000). The arrow points to the expected size of the protein. Each lane of each CLIP experiment represents a replicate of independently grown cells.