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. 2023 Jul 19;9:50. doi: 10.1038/s41522-023-00418-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Volcano plot depicting differentially expressed genes (blue and red dots) based on RNA-Seq and global transcriptome analyses. Downregulated genes with a log2FC of -1 or lower are depicted in red, and upregulated genes with a log2FC of 1 or higher are depicted in blue. Black dots represent genes not differentially expressed. b Significantly upregulated and downregulated genes were categorized according to known or predicted function. The graph shows the number of genes that fall into each category. Blue bars correspond to upregulated genes and red bars, downregulated genes. c, d Heatmaps displaying significantly downregulated genes for iron acquisition/homeostasis (c) and upregulated genes related to the DNA damage response (d) in the pulcherrimin mutant relative to wild type. RNA-seq transcriptome analysis was performed in samples from pellicle biofilms (collected at 72 h). Values are the average of three biological replicates per strain.