Fig. 7. Pulcherrimin production reduces ROS accumulation.

a Accumulation of different ROS species (H₂O₂, peroxynitrite, and/or hydroxyl radicals) in the pulcherrimin mutant and the wild type was visualized by using a ROS detection kit. Overnight cultures of the pulcherrimin mutant and the wild type growing in LBGM supplemented with 0.2 mM FeCl₃ were harvested, cells were stained with the ROS dye, and after incubation, images were taken under a fluorescence microscope. Scale bar, 10 μm. b Fluorescence quantification of the cells in the images (in a) was performed using the MicrobeJ plugin of Image J. Each dot in the violin plot corresponds to a single cell. The median values are represented by dashed horizontal lines. A statistically significant difference was observed between the wild type and the pulcherrimin mutant (p < 0.0001, ****).