Fig. 2. SHLP2 enhances iBAT thermogenesis and suppresses energy intake.

a–d Temporal changes (left) and average values (right) of O₂ consumption (a), CO₂ production (b), heat generation (c), and locomotor activity (d) from HFD-fed male mice with IP administration of either saline or SHLP2 (n = 6 each group). e Expression of thermogenic genes in iBAT from indicated groups (n = 5 each group). f Representative H&E (top) and UCP1 (bottom) staining from iBAT of HFD-fed male mice (n = 3 each group). g Mitochondrial DNA contents in the iBAT (n = 6 saline, n = 7 SHLP2). h Serum norepinephrine (NE) levels in indicated group (n = 10 each group). i Immunoblots (left) and relative densitometry (right) of UCP1, p-p38 MAPK, p-CREB expression in the iBAT samples (n = 4 each group). Data shown are representative of three independent experiments with similar results. j Daily food intake of HFD-fed male mice treated with either saline or SHLP2 (n = 6 saline, n = 7 SHLP2). k Gene expression of neuropeptides regulating feeding in the hypothalamic samples (n = 5 each group). Scale bars, 50 µm. n indicates the number of biologically independent animals examined. Data were presented as mean ± SEM. Two-tailed Student’s t tests were used in bar graphs, and two-way ANOVA with Bonferroni post-hoc tests were used in line graphs. Source data are provided as a Source Data file.