Fig. 4. Central SHLP2 regulates energy homeostasis.

a, b Cumulative food intake (a) and percent body weight change (b) after an ICV administration of saline or SHLP2 (n = 8 saline, n = 6 SHLP2). c–f Temporal changes (left) and average value (right) of O₂ consumption (c), CO₂ production (d), heat generation (e), and locomotor activity (f) of male mice after an ICV injection of saline or SHLP2 (n = 5 each group). g Expression of thermogenic genes in the iBAT (n = 4 each group). h Immunoblots (top) for UCP1 and relative densitometry (bottom) in the iBAT (n = 3 saline, n = 5 SHLP2). Data shown are representative of three independent experiments with similar results. i, j GTT (i) and ITT (j) were performed in NC-fed male mice after 4 hrs of ICV administration of saline or SHLP2 (n = 4 each group). n indicates the number of biologically independent animals examined. Data were presented as mean ± SEM. Numbers of animals examined were expressed in parenthesis. Two-tailed Student’s t tests were used and two-way ANOVA with Bonferroni post-hoc tests were used in (c–f), (i). Source data are provided as a Source Data file.