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. 2023 Jul 19;14:4331. doi: 10.1038/s41467-023-40107-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Control or CA-IKK2^ON mice were infected with X31(1000 pfu) and ±DOX day 5–30 p.i. Influenza-specific CD8⁺ T cells (Db-NP_366–374 or Db-PA_224–233 tetramers⁺) in the lung parenchyma (T_RM). b Representative plots of CD69⁺ cells among lung NP₃₆₆-₃₇₄⁺ CD8⁺ T cells. Frequencies (%) and numbers (#) of NP_366–374⁺ CD8⁺ T cells at day 10 (top graph) and day 30 (bottom) p.i. Day 10: n = 5, control; n = 6 CA-IKK2^ON; Day 30: n = 10, control and CA-IKK2^ON mice pooled from 3 independent experiments. c % and # of CD69⁺ (left and middle) and CD69⁻ (right) cells among the lung NP_366–374 specific CD8⁺ T_RM at day 10 (left) and day 30 (right) p.i. Day 10, for %: n = 6 (Control and CA-IKK2ON); for #: n = 4 (control), n = 5 (CA-IKK2^ON). For Day 30 CD69^+/− cells: for % of n = 4, (control), n = 6 (CA-IKK2^ON); for # n = 5 mice from 2 independent experiments. d Numbers of CD103⁺ cells among the CD69⁺ cells. NP specific (left) and PA specific (right) at day 30 p.i. For NP: n = 4, control; n = 6 CA-IKK2^ON; For PA₂₂₄: n = 5, control; n = 6 CA-IKK2^ON mice from 2 independent experiments. e CXCR3 expression in CD69⁻ and CD69⁺ populations among the NP-specific T_RM at day 30 p.i. n = 3, control; n = 4, CA-IKK2^ON mice from 2 independent experiments. f % and # of circulating NP specific CD8⁺ T cells at day 30 p.i. For %: n = 14, control; n = 6 CA-IKK2^ON; For #: n = 9, control; n = 7 CA-IKK2^ON mice from 3 independent experiments. g Expression of the indicated T_RM-associated transcription factors and CD122 in influenza-specific CD8⁺ T_RM at 30 d.p.i. Control: n = 7 (Runx3 and Nur77), n = 8 (Eomes), n = 5(CD122); for CA-IKK2^ON and DN-IKK2^ON n = 4 mice from 3 independent experiments. h Eomes and Runx3 expression was determined in CD69^+/− cells among the NP-specific CD8⁺ T_RM at 30 d.p.i. as indicated. CD69: n = 5, 7, 6 (control; CD69⁺, CD69⁻). Eomes: n = 11, control; CD69^+/−: n = 6 mice pooled from 2 independent experiments. Bars represent mean values ± SD. P-values were determined by two-tailed unpaired t test, n.s. not significant. Source data provided as a source data file.