Table 1.
Primers used for genetic manipulation in this study.
| Primer number | Primer name | Sequence |
|---|---|---|
| 1 | nirK 5F1 | CTTATGGATCACTGAGGAGC |
| 2 | nirK MFR-5R | TTTGTTCAGCGGCTTGTAGTCGCTCTCCTTGTCTTAGG |
| 3 | gent MF | TACAAGCCGCTGAACAAA |
| 4 | gent MR | GAAACCAAGCCAACCAGG |
| 5 | nirK 3R1 | GCACCTTATCCAACACGC |
| 6 | nirK MRR-3F | CCTGGTTGGCTTGGTTTCGACCATAGGAGAGCACAATG |
| 7 | nirK 5F2 | GCAATGTGGTACCTGTGAG |
| 8 | nirK 3R2 | CGGATTAAGGCTAAAGTGGA |
| 9 | norD 5F1 | GCCCCAACAACACTAGAAC |
| 10 | norD MFR-5R | TTTGTTCAGCGGCTTGTACATGCGTCACATCTTATCG |
| 11 | norC 3R1 | GCGGAGACATTCTATTTGC |
| 12 | norC MRR-3F | CCTGGTTGGCTTGGTTTCGTTGATCGCTTGGTGATAG |
| 13 | norC 3R2 | CGGACAGCTATTTGGAATTG |
| 14 | norD 5 F2 | GATAACCTCACGCAGGATTAC |
| 15 | nnrS-5F | GTACAAAAAAGCAGGCTCCGAATTCGCCCTTACCGCTGACACCTAGCATCAGATCGC |
| 16 | nnrS-5R | GCATTACAGTTTACGAACCGAACAGGCTTATGTCAACACGTTGTCAGAGCCGAGACCCG |
| 17 | nirV-3F | GCCTTCTATCGCCTTCTTGACGAGTTCTTCTGACCTTAATCCGGGCGCACCGC |
| 18 | nirV-3R | CTTTGTACAAGAAAGCTGGGTCGAATTCGCCCTCCACGCCAATCTGAGCAATCCG |
| 19 | F-PC-Km | TTGACATAAGCCTGTTCGGTTCG |
| 20 | R-Km | TCAGAAGAACTCGTCAAGAAGGCG |