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. 2023 Jul 22;60:2023017. doi: 10.2141/jpsa.2023017

Table 5.   Changes in plasma concentrations of Nτ-methylhistidine and Nπ-methylhistidine in fasted and re-fed chickens.

Fasting Re-feeding
0 h 12 h 24 h 36 h 48 h
Body weight 1337.37 ± 35.96 B 1282.27 ± 38.27 C 1245.43 ± 38.24 D 1353.67 ± 38.58 B 1386.42 ± 42.73 A
Nτ-Methylhistidine
LC–MS/MS
(m/z 170.20>96.10)
6.72 ± 0.76 C 11.34 ± 0.48 B 14.60 ± 1.03 A 12.29 ± 1.18 B 11.89 ± 1.12 B
LC–MS/MS
(m/z 170.20>124.10)
7.02 ± 0.78 C 11.69 ± 0.51 B 14.87 ± 1.06 A 12.67 ± 1.19 B 12.41 ± 1.23 B
UHPLC 6.18 ± 0.92 B 9.70 ± 0.89 A 11.93 ± 1.43 A 10.56 ± 1.26 A 10.35 ± 1.42 A
Nπ-Methylhistidine
LC–MS/MS
(m/z 170.20>96.10)
6.19 ± 0.62 B 8.77 ± 0.53 A 9.21 ± 0.63 A 9.55 ± 0.71 A 8.73 ± 0.82 A

Results are expressed as the mean ± standard error (n = 8). Means with the same superscript letter within rows are not significantly different at P < 0.05.