Table 5. Changes in plasma concentrations of Nτ-methylhistidine and Nπ-methylhistidine in fasted and re-fed chickens.
| Fasting | Re-feeding | ||||||
| 0 h | 12 h | 24 h | 36 h | 48 h | |||
| Body weight | 1337.37 ± 35.96 B | 1282.27 ± 38.27 C | 1245.43 ± 38.24 D | 1353.67 ± 38.58 B | 1386.42 ± 42.73 A | ||
| Nτ-Methylhistidine | |||||||
| LC–MS/MS (m/z 170.20>96.10) |
6.72 ± 0.76 C | 11.34 ± 0.48 B | 14.60 ± 1.03 A | 12.29 ± 1.18 B | 11.89 ± 1.12 B | ||
| LC–MS/MS (m/z 170.20>124.10) |
7.02 ± 0.78 C | 11.69 ± 0.51 B | 14.87 ± 1.06 A | 12.67 ± 1.19 B | 12.41 ± 1.23 B | ||
| UHPLC | 6.18 ± 0.92 B | 9.70 ± 0.89 A | 11.93 ± 1.43 A | 10.56 ± 1.26 A | 10.35 ± 1.42 A | ||
| Nπ-Methylhistidine | |||||||
| LC–MS/MS (m/z 170.20>96.10) |
6.19 ± 0.62 B | 8.77 ± 0.53 A | 9.21 ± 0.63 A | 9.55 ± 0.71 A | 8.73 ± 0.82 A | ||
Results are expressed as the mean ± standard error (n = 8). Means with the same superscript letter within rows are not significantly different at P < 0.05.