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. 2023 Jul 4;136(13):jcs261026. doi: 10.1242/jcs.261026

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Individual spontaneous kiss-and-run and full fusion events. (A) Contrast-enhanced fluorescent images of single labeled Dilp2–FAP presynaptic sites in the absence of Ca²⁺ after application of MG–BTau fluorogen in OK6>Dilp2-FAP type Ib boutons. Upper panels labeled KR show consecutive images of kiss-and-run pore opening, whreas the lower panels labeled FF show consecutive images of full fusions. Numbers on images indicate times in seconds. Scale bar: 1 µm. (B,C) Normalized time courses of representative individual (B) kiss-and-run and (C) full fusion sites. Data representative of ten experiments.