Fig. 3.

cAMP increases the frequency of spontaneous full fusions. (A) Mean±s.e.m. frequency of kiss-and-run (KR, black bars) and full fusions (FF, white bars) in control boutons (Con, n=8 NMJs, 71 boutons, five animals), boutons treated with the cAMP agonist 8-parachlorophenyl-thio-cAMP (Cpt, n=7 NMJs, 61 boutons, four animals) or boutons treated with octopamine (Oct, n=11 NMJs, 141 boutons, five animals) in the presence of MG–BTau. Statistical comparisons are always between like-colored bars; **P<0.01, ***P<0.001 (one-way ANOVA followed by Dunnett's post test). (B) Mean±s.e.m. frequency of kiss-and-run (black bars) and full fusions (white bars) per bouton in DMSO control (Con, n=12 NMJs, 91 boutons, six animals) and FSK-treated neurons (FSK, n=17 NMJs 144 boutons, ten animals) in the presence of MG–BTau, **P<0.01, unpaired two-tailed t-test. (C) Frequency of kiss-and-run (black bars) and full fusions (white bars) in PACα-expressing boutons without illumination (–Light, n=11 NMJs, 92 boutons, four animals) and light-activated PACα (+Light, n=16 NMJs, 167 boutons, eight animals) in the presence of MG–BTau. Statistical comparisons are always between like-colored bars; *P<0.05, unpaired two-tailed t-test. There were no significant differences between kiss-and-run fusion frequency (black bars) in the presented experiments, unpaired two-tailed t-test. All data derived from Ib boutons of OK6>Dilp2–FAP animals. 1/min, min⁻¹.