Skip to main content
. 2023 Jul 4;136(13):jcs261026. doi: 10.1242/jcs.261026

Fig. 4.

Fig. 4.

Epac is not involved in cAMP-induced full fusions. (A) Frequency of full fusions (FF) in control OK6>Dilp2–FAP boutons (Con, n=8 NMJs, 63 boutons, four animals) and pretreated with Epac activator 8-(4-methoxyphenylthio)-2′-O-methyl-cAMP (Me, n=8 NMJs, 58 boutons, four animals). No significant difference was detected with unpaired two-tailed t-test. (B) Frequency of full fusions in NMJs treated with Epac inhibitor ESI-09 without FSK (−FSK, n=10 NMJs, 112 boutons, five animals) and with FSK (+FSK, n=7 NMJs, 55 boutons, four animals). ***P<0.001 (unpaired two-tailed t-test). (C) Frequency of full fusions in Epac-null flies without FSK (−FSK, n=11; 127 boutons, six animals) and with FSK (+FSK) n=17 NMJs, (193 boutons, nine animals), ***P<0.001 (unpaired two-tailed t-test). (D–F) Frequency of kiss-and-run fusions with conditions corresponding to A–C. Note that there were no significant differences in kiss-and-run fusions for the experimental conditions presented here. 1/min, min−1.