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. 2023 Jul 4;136(13):jcs261026. doi: 10.1242/jcs.261026

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Complexin participates in cAMP-evoked full fusions and neuropeptide release. (A) Release indicated by loss of ANF–GFP fluorescence (F) is induced by a 1 min 70 Hz stimulation (indicated by the horizontal bar) in elav-GAL4 UAS-ANF-GFP;;UAS-Cpx^S126A, Cpx^SH1 animals. n=4 NMJs (one bouton each per animal). (B) Forskolin-evoked ANF–GFP release in 7 min is abolished in elav-GAL4 UAS-ANF-GFP;;UAS-Cpx^S126A, Cpx^SH1 animals. WT, wild type Cpx; PB Con, photobleaching control. n=4–6 NMJs (1 bouton each per animal). ****P<0.0001; ns, not significant (one-way ANOVA followed by Tukey's test). (C) Frequency of kiss-and-run (KR, black columns) and full fusions (FF, white columns) in boutons from elav-GAL4;UAS-Dilp2-FAP;UAS-Cpx^S126A, Cpx^SH1/Cpx^Delta2 flies in the absence and presence of forskolin (−FSK: n=7 NMJs, 53 boutons, four animals; +FSK: n=14 NMJs, 124 boutons, six animals). Cpx^SH1 and Cpx^Delta2 are null mutants. Note that forskolin does not have a statistically significant effect (ns, not significant by unpaired two-tailed t-test) on full fusion frequency (open bars). Likewise, no effect was produced on kiss-and-run frequency. 1/min, min⁻¹.