Fig. 3.

Promotor activity and specificity measured by luciferase assay in different proliferating cell types. (A–D) Proliferating HEK293 (A), C2C12 (B), HSKM-Ab1167 (C) and H9C2 (D) were transfected with the constructs encoding luciferase under control of respective promotors. Luciferase activity was measured on day 1, 2, 3 and 4 post transfection. All samples were normalized to 1μg total protein and datapoints represent mean values±SEMs using a log₁₀ scale (n = 4). Asterisks represent significant differences over 1–4 days within each promotor group (^*p < 0.05; ^**p < 0.01; ^***p < 0,001). (E) Overview of all significant differences (^*p < 0.05; ^**p < 0.01; ^***p < 0,001; ^****p < 0,0001; ns = not significant) derived by the promotor driven luciferase activity at day 1, 2, 3 and 4, respectively.