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. 2023 Jul 4;10(4):575–592. doi: 10.3233/JND-221574

Fig. 4.

Fig. 4

Long-term promotor activity in mouse and human differentiated myotubes. (A–C) Promotor activity in C2C12 mouse myotubes after 10 days of differentiation. (D–F) Promotor activity in human Ab1167 myotubes after 7 days of differentiation. (A,D) Luciferase expression under control of respective promotors, based on luminescence normalized to 1μg total protein. (B,E) Relative normalized gene expression of tEGFP encoded by promotor constructs was analysed by RT-PCR. Datapoints represent mean values±SEMs (n = 3). Asterisks represent significant differences (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). (C,F) Representative pictures of GFP expressing myotubes under control of respective promotors. (C) C2C12 myotubes, treated with EPS ten days post differentiation and (F) human Ab1167 myotubes without any EPS treatment after 7 days of differentiation. GFP expressing myotubes (green) were immunostained with anti-titin antibody (red). NT = non transfected control. Scale bars = 50μm.