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Determination of the enzymatically active domain of Ply187. PCR cloning was used to express the native ply187 gene and four fragments derived therefrom in E. coli. The solid bars represent the enzymatically active amidase domains, and the dotted bars indicate the C-terminal domains. The insert in pPL187-F2 corresponds to the DNA fragment initially cloned on plasmid pPL187.
