FIG. 6.

(Upper panels) Effect of hol187 expression on total cellular ATP content (left), and ATP release into growth medium (right), by E. coli cells harboring either pBluescript (control) or pHOL187. Gene expression was induced at time zero, and samples were taken and analyzed at the indicated time points. (Lower panels) Effect of the synthesis of Hol187 and mutated proteins on the release of β-galactosidase from E. coli W3110 cells into the medium (indicated by the blue zones around colonies) during growth on agar plates containing the chromogenic substrate X-Gal. (A) Control (W3110 without plasmid); (B) W3110(pHOL187); (C) W3110(pHOL187-S); (D) W3110(pHOL187-L); (E) W3110(pHOL187-Ch).