TABLE 1.
Bacterial strains, bacteriophages, and plasmids used throughout this study
| Strain, phage, or plasmid | Genotype or relevant properties | Source or reference |
|---|---|---|
| Strains | ||
| S. aureus 187 | Host for phage 187; assay for lytic activity | H. W. Ackermann |
| M. luteus WS2331 | Assay for lytic activity | Laboratory stock |
| E. coli | ||
| JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB) [F′ traD36 proAB lacIqZΔM15] | Laboratory stock |
| JM109(DE3) | ::DE3, T7 RNA polymerase under lacUV5 control | Promega |
| LE392 | F−hsdR514 supE44 supF58 lacY1 galK2 galT22 metB1 trpR55 (phage host; permissive for λgt11) | Promega |
| HB101 | F−hsdS20 supE44 recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 (phage host; nonpermissive for λgt11) | Promega |
| W3110 | F− λ− | ATCC 27325 |
| Phages | ||
| 187 | Wild type, B1 siphovirus; approx. 45-kbp double-stranded DNA genome | H. W. Ackermann |
| λ | Wild type | ATCC 23724-B2 |
| λgt11 | cIts857; Sam100; lac promoter for expression of cloned genes | Promega |
| λgt11::hol187 | hol187 inserted into EcoRI site of λgt11 | This work |
| λgt11::hol187-S | Modified hol187 (encoding Hol187 ΔM1L2) inserted into EcoRI site of λgt11 | This work |
| λgt11::hol187-L | Modified hol187 (encoding Hol187 M3L) inserted into EcoRI site of λgt11 | This work |
| λgt11::hol187-Ch | Modified hol187 (encoding Hol187 V7D G8D) inserted into EcoRI site of λgt11 | This work |
| Plasmids | ||
| pSP72 | 2.4-kb cloning and expression vector; T7 promoter; Ampr | Promega |
| pBluescript II (SK−) | 3.0-kb cloning and expression vector; lac promoter; Ampr | Stratagene |
| pPL187 | 1,112-bp AluI fragment from phage 187 ligated into SmaI site of pSP72 | This work |
| pPL187-F1 | 471-bp PCR product (N-terminal domain of Ply187) inserted into BamHI site of pSP72 | This work |
| pPL187-F2 | 891-bp PCR product (C-terminally truncated Ply187) inserted into BamHI site of pSP72 | This work |
| pPL187-F3 | 1,887-bp PCR product (complete Ply187) inserted into BamHI site of pSP72 | This work |
| pPL187-F4 | 421-bp PCR product (central portion of Ply187) inserted into BamHI site of pSP72 | This work |
| pPL187-F5 | 1,416-bp PCR product (N-terminally truncated Ply187) inserted into BamHI site of pSP72 | This work |
| pHOL187 | 188-bp PCR product (encoding Hol187) (Fig. 1) inserted into EcoRI site of pBluescript | This work |
| pHOL187-S | 182-bp PCR product (encoding Hol187 ΔM1L2) inserted into EcoRI site of pBluescript | This work |
| pHOL187-L | 188-bp PCR product (encoding Hol187 M3L) inserted into EcoRI site of pBluescript | This work |
| pHOL187-Ch | 188-bp PCR product (encoding Hol187 V7D G8D) inserted into EcoRI site of pBluescript | This work |