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Low expression of GIGYF1 inhibits cell metastasis and EMT process. (A) Knockdown GIGYF1 with small interfering RNA in MGC-803 cells. (B) The changes in the expression of GIGYF2,4EHP after knocking down GIGYF1. (C) Transwell assays to detect cell migration. (D) Detection of cell healing ability by wound healing assays. (E) Western blotting detected the change of EMT related protein expression after GIGYF1 knockdown. *p < 0.05, **p < 0.01, ***p < 0.001.
