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. 2022 Apr 20;166(2):249–264. doi: 10.1111/imm.13471

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© 2022 The Authors. Immunology published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(a) Flow cytometry of cell surface HLA‐A, B, and C as detected by antibody W6/32 compared to control second‐stage FITC anti‐IgG alone (ctrl). (b) NTA analysis of particles released by cell lines. One comparative graph of three recordings is shown, with mean and modes indicated. (c) Immunoblotting analysis of detergent cell lysates (CL) and EV lysates of all cell lines, probed for HLA‐B and C, CD9, CD63, CD81, and control proteins transporter associated with antigen processing (TAP1) and calnexin (CXN)