. 2022 Apr 20;166(2):249–264. doi: 10.1111/imm.13471

TABLE 3.

List of peptides matching with previously identified T‐cell epitopes from EV HLA‐I ligandomes of breast cancer, melanoma, and myeloma cell lines

Number	Antigen name	Parent protein	Epitope ID	Position	Uniprot entry name	PubMed ID	T‐cell epitope	HLA ‐I restriction	Peptides identified in the study	Cell line
1	Carcinoma‐associated mucin	P15941	T000434	130–138, multiple positions	MUC1_HUMAN	10361129	STAPPAHGV	HLA‐A02:01, HLA‐A11:01	STAPPAHGV	RPMI 8226
2	Ubiquitin‐conjugating enzyme E2 D2	P62837	243 855	59–66	UB2D2_HUMAN	22869377	YPFKPPKV	HLA‐B*51	YPFKPPKVTF*	HCC 1954
2	Ubiquitin‐conjugating enzyme E2 D2	P62837	243 855	59–66	UB2D2_HUMAN	22869377	YPFKPPKV	HLA‐B*51	YPFKPPKV	HCC 1806
3	Chromatin Assembly Factor 1 Subunit A	Q13111	T001077	772–781	CAF1A_HUMAN	24048523	SPRSPSTTYL	HLA‐B*07:02	SPRSPSTTYL	ESTDAB‐056
4	Clathrin heavy chain 1	Q00610	442 923	311–320	CLH1_HUMAN	22869377	ATAGIIGVNR	HLA‐A*11	ATAGIIGVNR	HCC 1806
5	G1/S‐specific cyclin‐D1	P24385	T000697	115–124	CCND1_HUMAN	12384544	ETIPLTAEKL	HLA‐A*68:01	ETIPLTAEKL	HCC 1806
6	Cytochrome P450 1B1	Q16678	T000701	5–13	CP1B1_HUMAN	12869499	FLDPRPLTV	HLA‐A*02:01	FLDPRPLTV	MDA‐MB‐231
7	E3 ubiquitin‐protein ligase Mdm4	Q00987	236 802	273–281	MDM2_HUMAN	25548167	DEVYQVTVY	HLA‐B*18	DEVYQVTVY	MDA‐MB‐415
8	Fructose‐bisphosphate aldolase A	P04075	2874	216–224	ALDOA_HUMAN	11782012	ALSDHHIYL	HLA‐A*02:01	ALSDHHIYL	MCF7
9	Histone H3.3	P84243	T000946	59–67	H33_HUMAN	16196104	ELLIRKLPF	HLA‐B*08	ELLIRKLPF	HCC 1395
9	Histone H3.3	P84243	T000946	59–67	H33_HUMAN	16196104	ELLIRKLPF		ELLIRKLPF	MDA‐MB‐453
10	HER2 receptor	P04626	67 385	63–71	ERBB2_HUMAN	17397516	TYLPTNASL	HLA‐A*24	TYLPTNASLSF*	HCC 1954
11	Mammaglobin‐A	Q13296	64 399	32–40	SG2A2_HUMAN	15538043	TINPQVSKT	HLA‐A*02	KTINPQVSKTEY*	MDA‐MB‐415
12	Poly (ADP‐ribose) polymerase family, member 12	Q9H0J9	T000954	669–677	PAR12_HUMAN	16033845	VYPEYVIQY	HLA‐C*07:02	VYPEYVIQY	ESTDAB‐056
13	Poly(RC) Binding Protein 2	Q15366	T001070	183–191	PCBP2_HUMAN	24048523	RPKPSSSPV	HLA‐B*07:02	RPKPSSSPVIF*	ESTDAB‐056
14	Similar to retinoblastoma‐binding protein 4, partial	Q09028	215 983	245–253	RBBP4_HUMAN	22869377	NLKLKLHSF	HLA‐B*57	NLKLKLHSF	HCC 1395
15	40S ribosomal protein SA	P08865	T000873	146–154	RSSA_HUMAN	16709854	ALCNTDSPL	HLA‐A*02:01	NTDSPLRY*	MDA‐MB‐361
									NTDSPLRY*	MDA‐MB‐453
									NTDSPLRY*	MCF7

Note: Peptides showing partial match with known T‐cell epitopes are marked with an asterisk (*). Epitope IDs are the reference number of T‐cell epitope listed on Tantigen (IDs beginning with T) and IEDB (IDs beginning with number).