. 2022 Apr 20;166(2):249–264. doi: 10.1111/imm.13471

TABLE 4.

Shortlist of peptides derived from TAA proteins (TAApep) detected in EV HLA‐I ligandome of breast cancer, melanoma, and myeloma cell lines

Number	Antigen full name	Antigen name	Antigen accession	Protein accession	Uniprot protein entry	Number of peptides	Peptide	Cell lines
1	Canalicular multispecific organic anion transporter 2	ABCC3	Ag000410	O15438	MRP3_HUMAN	2	AEKAFVSV	HCC 1395
1	Canalicular multispecific organic anion transporter 2	ABCC3	Ag000410	O15438	MRP3_HUMAN	2	AYLHTTTTF	MDA‐MB‐361
2	Ataxin‐2‐like protein	ATXN2L	Ag004315	Q8WWM7	ATX2L_HUMAN	1	AHYPSQPVF	HCC 1806, RPMI 8226 8226
3	G1/S‐specific cyclin‐D1	CCND1	Ag000285	P24385	CCND1_HUMAN	4	NYLDRFLSL	HCC 1954
							AEETCAPSV	HCC 1395
							EVFPLAMNY	ESTDAB‐056
							EVFPLAMNYL	ESTDAB‐056
4	Cell division control protein 45 homologue	CDC45	Ag004342	O75419	CDC45_HUMAN	1	RPVNVVNVY	HCC 1954
5	Cleavage and polyadenylation specificity factor subunit 1	CPSF1	Ag000125	Q10570	CPSF1_HUMAN	2	SVLPAYLSY	HCC 1395
5	Cleavage and polyadenylation specificity factor subunit 1	CPSF1	Ag000125	Q10570	CPSF1_HUMAN	2	ETVSGLKGY	ESTDAB‐056
6	Catenin beta‐1	CTNNB1	Ag000058	P35222	CTNB1_HUMAN	2	HPPSHWPLI	HCC 1806
							AQNAVRLHY	HCC 1806
7	Receptor tyrosine‐protein kinase erbB‐2	ERBB2	Ag000001	P04626	ERBB2_HUMAN	2	TPTAENPEY	HCC 1954
7	Receptor tyrosine‐protein kinase erbB‐2	ERBB2	Ag000001	P04626	ERBB2_HUMAN	2	MPNPEGRYTF	HCC 1954
8	ets variant 5	ETV5	Ag004221	P41161	ETV5_HUMAN	1	KVAGERYVY	ESTDAB‐056
9	Neutral alpha‐glucosidase AB	GANAB	Ag004500	Q14697	GANAB_HUMAN	1	AVAAVAARR	HCC 1806
10	Glycoprotein NMB	GPNMB	Ag000250	Q14956	GPNMB_HUMAN	1	STINYKWSF	ESTDAB‐056
11	Transcription factor HIVEP2	HIVEP2	Ag004270	P31629	ZEP2_HUMAN	1	SPLIRSNSV	HCC 1395
12	Heme oxygenase (decycling) 1	HMOX1	Ag000461	P09601	HMOX1_HUMAN	1	EVIPYTPAM	ESTDAB‐056
13	Heterogeneous nuclear ribonucleoprotein L	HNRNPL	Ag000394	P14866	HNRPL_HUMAN	2	VEFDSVQSA	HCC 1954
13	Heterogeneous nuclear ribonucleoprotein L	HNRNPL	Ag000394	P14866	HNRPL_HUMAN	2	IYIAGHPAF	HCC 1806
14	Heat shock 70 kDa protein 1B	HSPA1B	Ag000092	P0DMV9	HS71B_HUMAN	2	KQTQIFTTY	HCC 1806
14	Heat shock 70 kDa protein 1B	HSPA1B	Ag000092	P0DMV9	HS71B_HUMAN	2	TVFDAKRLIGR	HCC 1806
15	Heat shock protein beta‐1	HSPB1	Ag004328	P04792	HSPB1_HUMAN	1	NEITIPVTF	MDA‐MB‐415
16	Insulin‐like growth factor 2 mRNA binding protein 3	IGF2BP3	Ag000506	O00425	IF2B3_HUMAN	1	ETAVVNVTY	ESTDAB‐056
17	Microtubule‐Actin cross‐linking factor 1 isoforms 1/2/3/5	MACF1	Ag004285	Q9UPN3	MACF1_HUMAN	3	EEAFHQGLISA	HCC 1395
							AEKFWYDMA	HCC 1395
							NQKPPSAEY	RPMI 8226 8226
18	Melanoma‐associated antigen C2	MAGEC2	Ag000036	Q9UBF1	MAGC2_HUMAN	1	NAVGVYAGR	HCC 1806
19	E3 ubiquitin‐protein ligase Mdm2 (Fragment)	MDM2	Ag000287	Q00987	MDM2_HUMAN	1	DEVYQVTVY	MDA‐MB‐415
20	ATPase MORC2	MORC2	Ag004287	Q9Y6X9	MORC2_HUMAN	1	IETELIYKY	MDA‐MB‐415
21	Nucleolar Protein Interacting With The FHA Domain Of MKI67	NIFK	Ag004290	Q9BYG3	MK67I_HUMAN	1	SQFGTVTRF	RPMI 8226 8226
22	2′‐5′‐oligoadenylate synthase 3	OAS3	Ag000403	Q9Y6K5	OAS3_HUMAN	2	TVLELVTQY	HCC 1954
22	2′‐5′‐oligoadenylate synthase 3	OAS3	Ag000403	Q9Y6K5	OAS3_HUMAN	2	AEIISEIRA	HCC 1395 EV
23	Serine/threonine‐protein kinase PAK 2	PAK2	Ag000472	Q13177	PAK2_HUMAN	1	NENPLRALY	MDA‐MB‐415
24	Protein mono‐ADP‐ribosyltransferase PARP12	PARP12	Ag000523	Q9H0J9	PAR12_HUMAN	2	EYQKVWNLF	HCC 1954
							DEFGSWQEY	MDA‐MB‐415
25	Poly(rC)‐binding protein 2 (Fragment)	PCBP2	Ag004321	Q15366	PCBP2_HUMAN	1	LEGPPLEAY	MDA‐MB‐415
26	Serine/threonine‐protein phosphatase PP1‐alpha catalytic subunit	PPP1CA	Ag004293	P62136	PP1A_HUMAN	1	KYPENFFLL	HCC 1954
27	Replication protein A 70 kDa DNA‐binding subunit (Fragment)	RPA1	Ag000443	P27694	RFA1_HUMAN	2	AEAILGQNAA	HCC 1395
27	Replication protein A 70 kDa DNA‐binding subunit (Fragment)	RPA1	Ag000443	P27694	RFA1_HUMAN	2	KVIDQQNGLY	MDA‐MB‐415
28	Protein SON	SON	Ag004333	P18583	SON_HUMAN	3	SAYERSMM	HCC 1395
							YTDSYTDTY	MDA‐MB‐453
							SPMAERSMM	ESTDAB‐056
29	Serine/Arginine Repetitive Matrix 2	SRRM2	Ag004276	Q9UQ35	SRRM2_HUMAN	1	SPRKPIDSL	ESTDAB‐056
30	Signal transducer and activator of transcription	STAT1	Ag000451	P42224	STAT1_HUMAN	3	EELEQKYTY	MDA‐MB‐415
							SEVLSWQF	MDA‐MB‐415
							DQYSRFSL	MDA‐MB‐415
31	STAGA complex 65 subunit gamma	SUPT7L	Ag000225	O94864	ST65G_HUMAN	1	GSSPVFNQR	HCC 1806
32	Tensin‐3	TNS3	Ag004302	Q68CZ2	TENS3_HUMAN	3	YITERIIAV	HCC 1395
							QQMVVAHQY	MDA‐MB‐415
							TERIIAVSF	MDA‐MB‐415
33	DNA topoisomerase 2‐alpha	TOP2A	Ag000436	P11388	TOP2A_HUMAN	1	ATKTKFTM	HCC 1395
34	Topoisomerase (DNA) II beta 180 kDa	TOP2B	Ag000441	Q02880	TOP2B_HUMAN	1	SPRYIFTML	ESTDAB‐056
35	Targeting protein for Xklp2	TPX2	Ag004304	Q9ULW0	TPX2_HUMAN	1	KSSDQPLTV	HCC 1954
36	Thymidylate synthase	TYMS	Ag000483	P04818	TYSY_HUMAN	1	DAHIYLNHI	HCC 1806
37	Serine/threonine‐protein kinase WNK2 (Fragment)	WNK2	Ag000399	Q9Y3S1	WNK2_HUMAN	2	HESDVKIVA	HCC 1954
37	Serine/threonine‐protein kinase WNK2 (Fragment)	WNK2	Ag000399	Q9Y3S1	WNK2_HUMAN	2	QEHVPTSSA	HCC 1954
38	Zinc Finger CCCH‐Type Containing 14	ZC3H14	Ag004311	Q6PJT7	ZC3HE_HUMAN	1	KTTNVRQTY	ESTDAB‐056

Note: The shortlist is selected for TAAs which have at least one immunogenic peptide defined in Tantigen database.