FIG. 7.

TolRII-III dimerizes and does not interact with TolA and TolQ. TPS13(pQA), TPS13(pINRII-IIIM), and TPS13(pQA, pINRII-IIIM) cells were cross-linked for 20 min in the presence of 1% formaldehyde as described in Materials and Methods. TolRII-III production from pINRII-IIIM was induced for 30 min with 50 μM IPTG prior to cross-linking. Extracts of these cells, treated (F) or not treated (C) with formaldehyde, were analyzed by immunoblotting on an SDS–12.5% PAGE gel with the AbTolR antiserum, as described in the legend to Fig. 1. The positions of TolRII-III and TolRII-III dimer and their molecular masses are indicated on the right, and the positions of the molecular mass markers are indicated on the left.