FIG. 8.

TolRI-II dimerizes and interacts with TolQ, but not with TolA. TPS13(pARTRI-II), TPS13(pQA), and TPS13(pQA, pARTRI-II) cells were cross-linked for 20 min in the presence of 1% formaldehyde, as described in Materials and Methods. TolRI-II production from pARTRI-II was induced for 2 h with 0.5 mg of arabinose/ml prior to cross-linking. Extracts of these cells, treated (F) or not treated (C) with formaldehyde, were analyzed by immunoblotting on an SDS–10% PAGE gel with the AbTolR antiserum as described in the legend to Fig. 1. The positions of TolRI-II dimer and TolRI-II–TolQ complex and their molecular masses are indicated on the right.