FIG. 9.

TolRII-His dimerizes in vitro. Purified TolRII-His (10 μl; 1 mg/ml) was treated (F) or not treated with formaldehyde. The samples not treated with formaldehyde were heated (H) or not heated (NH) for 20 min at 96°C before being loaded on SDS-PAGE gels. After transfer to nitrocellulose, bands were revealed with the AbTolR antiserum as described in the legend to Fig. 1. The positions of TolRII-His, TolRII-His dimer, and TolRII-His trimer are indicated on the right. The positions of the molecular mass markers are indicated on the left.