Table 1. Observed Responses in Box–Behnken Trial Runs for EXE–GEN.

run	factor 1 A: % methanol	factor 2 B: autosampler temp	factor 3 C: flow rate mL/min	response 1 retention time (EXE) min	response 2 retention time (GEN)	response 3 tailing factor (EXE)	response 4 tailing factor (GEN)	response 5 theoretical plates (EXE)	response 6 theoretical plates (GEN)
1	30	15	1.2	2.07	1.69	1.47	1.57	7500	6200
2	40	15	1	2.11	1.62	1.41	1.58	7540	6232
3	30	35	1.2	2.19	1.7	1.47	1.45	7600	6297
4	30	25	1	2.15	1.68	1.56	1.57	7574	6271
5	20	15	1	2.09	1.66	1.48	1.55	7454	6174
6	30	15	0.8	2.09	1.69	1.48	1.56	7550	6252
7	40	25	1.2	2.15	1.67	1.39	1.49	7552	6255
8	30	25	1	2.14	1.67	1.55	1.56	7550	6249
9	30	25	1	2.13	1.68	1.57	1.55	7549	6258
10	40	25	0.8	2.14	1.64	1.45	1.54	7580	6282
11	30	25	1	2.15	1.67	1.54	1.56	7550	6251
12	20	25	1.2	2.13	1.66	1.45	1.54	7432	6139
13	20	35	1	2.2	1.65	1.46	1.55	7422	6125
14	30	25	1	2.15	1.67	1.55	1.58	7540	6241
15	40	35	1	2.21	1.64	1.45	1.42	7640	6344
16	20	25	0.8	2.15	1.69	1.44	1.56	7421	6131
17	30	35	0.8	2.18	1.69	1.49	1.53	7545	6247