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Northern blot detection of pagR and pagA mRNA. RNA samples (20 μg) from cells grown at 37°C in 5% CO2 were probed with biotinylated DNA probes corresponding to sequences internal to pagR (A) and pagA (B). Transcript sizes are indicated. (C) The relative locations of the pagA and pagR mRNA transcripts and the Ωkm-2 insertions in UT62 and UT119 are shown.
