SPR results of F-Id-3k, F-Id-3o, and PF74 binding to monomeric and hexameric CA.
| Compounds | K D a (μM) | Ratiob | k off c (1/s) | |
|---|---|---|---|---|
| Monomer | Hexamer | |||
| F-Id-3k | 12 ± 1.3 | 5.3 ± 0.55 | 2.2 | 0.25 ± 0.013 |
| F-Id-3o | 13 ± 2.3 | 5.2 ± 0.52 | 2.5 | 0.25 ± 0.045 |
| PF74 | 3.4 ± 1.3 | 0.16 ± 0.041 | 21.4 | 0.018 ± 0.0039 |
a
All values represent the average response from three replicates. Errors represent standard deviation (SD).
b
Ratio = KMonomerD/KHexamerD highlights the preference for HIV-1 CA monomer or hexamer binding.
c
k off = off-rate measured for HIV-1 CA hexamer.