Table 2.
Antibodies
| Antigen | Clone | Dilution | Antigen retrieval | Source |
|---|---|---|---|---|
| CD45 | PD7-26,2B11 | 1:200 | Microwave/citrate | Dako, Carpinteria, CA |
| CD68 | PG/M1 | 1:50 | Pronase | Dako |
| MAC-1 | HAM56 | 1:1000 | Proteinase K | Dako |
| MHC-class ll | CR3/43 | 1:50 | Heat/high pH Dako | Dako |
| MRP14 | BMA-S36,48 | 1:25 | Microwave/citrate | Accurate Chemical, Westbury, NY |
| RCA-1 lectin | 1:4000 | Vector, Burlingame, CA | ||
| CD209 DC-SIGN | 1:100 | Heat/high pH Dako | R&D Systems, Minneapolis, MN | |
| IgG | 1:8000 | Proteinase K | Dako | |
| C9neo (MAC) | B7 | 1:20 | Proteinase K | BP Morgan, Cardiff University, Cardiff, UK |
| CD3 | 1:50 | Microwave/citrate | Dako | |
| CD4 | 1:10 | Microwave/citrate | Novocastra, Newcastle Upon Tyne, UK | |
| CD8 | C8/144B | 1:100 | Heat/high pH Dako | Dako |
| CD45RO | UCHL1 | 1:150 | Microwave/citrate | Novocastra |
| PCNA | PC10 | 1:80 | Dako | |
| CD16 | 2H7 | 1; 30 | Microwave/citrate | Novocastra |
| AQP4 | 1:500–1000 | Microwave/citrate | Millipore Corp, Billerica, MA |