Table 2.

Proliferation and cell invasion assays in T47D and MCF-7 cells

	Proliferationa		Invasiona
	T47Db	MCF-7b	T47D	MCF-7
Control c	1.00 ± 0.00 (n = 15)	1.00 ± 0.00 (n = 17)	1.00 ± 0.00 (n = 15)	1.00 ± 0.00 (n = 9)
WT	0.99 ± 0.00 (n = 15)	0.98 ± 0.03 (n = 17)	2.43 ± 0.06** (n = 15)	1.05 ± 0.09 (n = 9)
WT + m1	1.03 ± 0.01 (n = 15)	0.99 ± 0.01 (n = 17)	1.60 ± 0.07*, ** (n = 15)	0.88 ± 0.14 (n = 9)
WT + m2	1.02 ± 0.01 (n = 15)	1.01 ± 0.02 (n = 17)	0.85 ± 0.04* (n = 15)	2.25 ± 0.10*, ** (n = 9)
WT + m5	1.03 ± 0.02 (n = 15)	1.03 ± 0.04 (n = 17)	2.14 ± 0.04*, ** (n = 15)	1.07 ± 0.14 (n = 9)

*p < 0.01 compared to wild-type GATA3; **p < 0.005 compared to control

^aProliferation and invasion for WT and mutant GATA3 constructs were expressed as fold change with respect to control transfection with pcDNA3.1 alone. Mean ± standard error of the mean (n = number of experiments) are shown

^bThe T47D and MCF-7 are ER-positive breast cancer cell lines; the MCF-7 cells have a GATA3 mutation, D336fs, whereas the T47D cells do not have any GATA3 abnormalities

^cControl DNA (pcDNA3.1), WT – wild-type GATA3, and mutant GATA3 ( m1, m2, and m5 mutations—Table 1) constructs