Fig. 5.

IRAK-1/4 inhibition does not block NFκB constitutive activity in KGN and COV434 cells. to assess IRAK-1/4 blockade on NFκB activity a KGN and b COV434 cells were transiently transfected with 0.5 μg of either pTAL-Luc (gray bar) or pNFκB-Luc (black bar) for 24 h. Transfected cells were treated with either vehicle, TNFα (10 ng/ml), IL-1α (10 ng/ml), and/or IRAK-1/4 inhibitor (5 μM) for 24 h. Firefly luciferase activity was measured and expressed as arbitrary units relative to vehicle-treated cells. The results from four separate experiments, each of which was performed in sextuplicate, are shown as mean ± SEM. ***p < 0.001; ns, not significant