Fig. 6.

Effects of SDHB mutations on migration of neuroblastoma clones. Wound-healing migration assay: confluent and serum-starved cells were scratched with a micropipette tip and photographed (time T = 0). Cells were challenged with 1 % FCS, and after 24 h, the wounds were photographed again (time T = 24 h) to visualize the incoming cells. The experiment was repeated four times with similar results. Cell-free pixels, representing the wounds, were measured both at T = 0 and T = 24 h. The difference, expressed as percentage, represents the healing. In the figure are shown the representative pixels values, for each clone; experiments were repeated four times. *p < 0.05 compared to controls (pcDNA and wt-transfected clones)