Table 2.
Analysis of mitochondria
| Clone | % of mitochondria | % of mitochondria areas (ma) | % of internal cristae areas (ica) | ma/ica ratio |
|---|---|---|---|---|
| pcDNA | 17 ± 0.2 | 100 ± 24 | 100 ± 15 | 6 |
| SDHB wt | 18 ± 2 | 101 ± 22 | 101 ± 13 | 5.9 |
| SDHB p.Cys101Tyr | 17 ± 3 | 91 ± 24 | 99 ± 16 | 5.5 |
| SDHB p.Cys191Tyr | 19 ± 4 | 124 ± 22 | 104 ± 7 | 7.2 |
| SDHB p.Ser163Pro | 18 ± 3 | 129 ± 21 | 119 ± 18 | 6.5 |
Briefly, to analyse the number of mitochondria, five cytoplasmic fields for single clone were chosen at random, and a count by point was performed. Areas of mitochondria and areas of cristae were measured using iTEM image analysis software (SIS). Mitochondrial swelling/disruption was estimated by the ratio between the surface area and overall areas of cristae