Fig. 3.
Effects of unmodified tetrac and Tetrac NP on abundance of tumor-relevant mRNAs or proteins in PANC-1 cells. a Cells were cultured for 2 days in the presence of control solvent, tetrac (10−7 M), or Tetrac NP (10−8 M). Harvest of total RNA and PT-PCR was performed as described in “Materials and Methods” section. Unmodified tetrac and Tetrac NP both increased abundance of p21 and p53 mRNAs (P < 0.05). Neither agent significantly affected expression of EGFR. GAPDH was the internal control used to correct IOD in graphed data. b Cells were cultured for 3 days in the presence of control solvent, unmodified tetrac, and Tetrac NP, as in (a). Immunoblots of total proteins harvested from the samples were prepared. Increased accumulation of THBS1 protein was obtained with tetrac (P = 0.029) and Tetrac NP (P < 0.022). Tetrac, but not Tetrac NP, increased accumulation of XIAP (P = 0.006). β-Actin was the internal control used to correct IOD in graphed data (*P < 0.05 and **P < 0.01). Experiments in (a) and (b) were performed three times. Effects of unmodified tetrac and Tetrac NP on abundance of tumor-relevant mRNAs or proteins in MPanc96 cells. c Cells were cultured for 2 days in the presence of control solvent, tetrac (10−7 M), or Tetrac NP (10−8 M). Harvest of total RNA and PT-PCR was performed as described in “Materials and Methods” section. Unmodified tetrac and Tetrac NP both increased expression of p21 and p53 mRNAs (P < 0.05). Tetrac NP (P < 0.01) but not tetrac significantly reduced the expression of EGFR. GAPDH was the internal control. d Cells were cultured for 3 days in the presence of control solvent, unmodified tetrac, and Tetrac NP, as in (a). Immunoblots of total proteins harvested from the samples were prepared. Increased accumulation of THBS1 protein was obtained with tetrac (P < 0.05) and Tetrac NP (P < 0.01). Tetrac significantly increased accumulation of XIAP (P < 0.05), while Tetrac NP decreased accumulation of XIAP (P < 0.01). β-Actin was the internal control for correction of IOD (*P < 0.05 and **P < 0.01). Experiments in (c) and (d) were carried out three times