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. 2023 May 15;12(13):14452–14467. doi: 10.1002/cam4.6074

FIGURE 2.

FIGURE 2

USP10 affects the invasion and migration of OS cells. (A) Western blotting and qRT‐PCR experiments of USP10 protein and mRNA levels in U2‐OS and 143‐B cell lines transfected with shNC and shUSP10 plasmids. (B) P‐USP10 plasmid was used to transfect Saos‐2 and MG‐63 OS cell lines, western blot and qRT‐PCR confirmed USP10 rise. (C, E) Wound healing and transwell tests were utilized to identify the impact of knockdown USP10 in the migration and invasiveness of U2‐OS and 143‐B (**p < 0.01; magnification, 200×, scale bar, 100 μm). (D, F). Migration and invasion of Saos‐2 and MG‐63 cells transfected with control and p‐USP10 were studied using wound healing and transwell assays (**p < 0.01, magnification, 200×, scale bar, 100 μm).