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. 2023 May 15;12(13):14452–14467. doi: 10.1002/cam4.6074

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© 2023 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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TGF‐β can block the inhibitory effect of shUSP10 on OS cell EMT. (A, B) The relative expression levels of EMT‐related mRNAs and proteins were identified by qRT‐PCR and western blotting after knockdown of USP10 in osteosarcoma cells and treatment with TGF‐β. (C–E) In osteosarcoma cells stably transfected with shNC/shUSP10 treated with TGF‐β, changes in migration and invasiveness capabilities were analyzed by transwell and wound healing tests (**p < 0.01, magnification, 200×, scale bar, 100 μm).