FIGURE 7.

Downregulation of USP10 can inhibit the growth of OS cells in vitro, inhibit the growth and metastasis in vivo. (A, B) EdU and CCK8 assays showed that shUSP10 cell proliferation was inhibited (*p < 0.05). (C, D) Nude mice were subcutaneously inoculated with osteosarcoma cells (143B) transfected with shUSP10 and shNC plasmids to observe tumor formation in vivo, and tumors were collected and weighed 30 days later (**p < 0.01). (E) Osteosarcoma cells (143B) were transfected with shUSP10 and shNC plasmids into the tail vein of nude mice, and lung tissues were collected for HE staining 30 days later. (F) Prevalence of lung metastases in shNC and shUSP10 groups of nude mice. (G) Representative photographs of the lungs in shNC and shUSP10 mice.